Application

Invertase from baker′s yeast (S. cerevisiae) has been used:in liquid media (YES) and is used to determine the invertase activitysupplemented to the reaction mixture in the extraction and analysis of sugars and starchfor sucrose hydrolysis

Used in the production of confectionary foods and artificial honey.

Biochem/physiol Actions

Invertase hydrolyzes sucrose into glucose and fructose yielding a colorless product, unlike acid hydrolysis which produces colored products.

General description

Invertase is encoded by the SUC2 (Saccharomyces cerevisiae S288C) gene. In yeast this enzyme exist in: glycosylated (secreted) and non-glycosylated (intracellular) form. The external invertase is composed of carbohydrate (50%) and 60,000 D subunits.

Packaging

100 mg in poly bottle

Unit Definition

One unit will hydrolyze 1.0 µmole sucrose to glucose and fructose per minute at pH 4.6 at 25 °C.